ABSTRACT
Aim To investigate the protective effect of resveratrol( Rev) on atherosclerosis( AS) , and clarify the rolcof Rev in regulating IncRNA MALATi (MALAT1) and the role of MALATI in AS. Methods ApoE'/ mice were randomly divided into two groups-, the high-fat diet(HFD) group, the HFD plus Rev treatment group, and the atherosclerosis model was established for 16 weeks. Hematoxylin-eosin( HE) staining was used to detect the area of aortic plaques in mice. And electron microscopy was performed to measure the formation of foam cells. The effects of Rev on MALATI, inflammatory cytokine ( 1L-6, 1L-1(3 and TNF-a) were examined using real time PCR. In addition, the roles of MALATI on inflammatory cytokine, and the influence of free cholesterol (FC) on the ex pression of MALATI were measured by real time PCR. Results The Rev treatment group significantly reduced arterial plaque areas and foam cell formation. In normal peritoneal macrophages, Rev could increase the expression of MALATI, and FC could down-regulate MALATI. Furthermore, Rev could decrease the expression of IL-6, 11,-1(3 and TNF-a in a dose-dependent manner. Knockdown of MALATI promoted the mRNA expression of IL-6 and TNF-a. Conclusions Rev may modulate the expression of inflammatory cytokine IL-6, lL-lp and TNF-a by affecting the level of MALAT, thereby exerting the anti-atherogenic effects.